Biochem/physiol Actions

Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation

General description

Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Stein, R.L., et al. 1996. Biochemistry 35, 3899.Ciechanover, A. 1994. Cell 79, 13.Dick, L.R., et al. 1994. J. Immunol. 152, 3884.Dick, L.R., et al. 1991. Biochemistry 30, 2725.Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.

Packaging

10 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)